Skip to content
ShodhX: Through Science, For Science

Optimizing protein expression in heterologous system: Strategies and tools

Journal
Meta Gene, published
DOI
10.1016/j.mgene.2021.100899
PubMed
Not indexed in PubMed

Citation figures are shown for reference only. They are not used to judge whether a study is standalone or a follow-up.

Assessment

Rule-based score: Standalone study (follow-up index 9/100, see the score card)

Score card

Calculated by fixed rules from the data on this page. Each line shows what earned the points. Citation counts are not used.

Follow-up index

9/100

Standalone study

55+ follow-up · 25 or less standalone · in between unclear

+0 Similar earlier article by the same group
none found
+0 Cites earlier studies by its own authors
0 studies
+0 Self-citations by ORCID iD
0 of 87 references (0%)
+0 Continuation wording
none
+9 Very similar earlier article by another group
60% keyword match: Strategies of codon optimization for high-level heterologous protein expression in microbial expression systems (2017)

Impact

21/100

Overall = 35% scientific advancement + 25% economic / commercial + 15% human resource development + 25% technologies & techniques

  • Scientific advancement47

    mentions “mechanism” · novelty (100 − follow-up index) × 0.3 = +27

  • Economic / commercial0

    no matching terms

  • Human resource development6

    no matching terms · 2 institutions, 4 authors = +6

  • Technologies & techniques15

    1 × 15: RNA and protein synthesis mechanisms

Keywords used for the assessment (20)

System studied

  • Heterologous
  • Gene
  • Codon usage bias
  • Gene expression
  • Polyadenylation
  • RNA splicing

Technologies & techniques

  • RNA and protein synthesis mechanisms

Topic

  • heterologous expression
  • codon optimization
  • Kozak sequence
  • Shine-Dalgarno sequence
  • GC content
  • mRNA stability
  • cryptic splice site
  • polyadenylation signal
  • synthetic gene design
  • genetic modification
  • expression vectors
  • Molecular Biology Techniques and Applications
  • Viral Infectious Diseases and Gene Expression in Insects

Self-citations

0 of 87 references (0%) share an ORCID iD with this article's authors. 0 share an author by any match. 4 of 4 authors have an ORCID iD.

Similar earlier articles

Published before this article and sharing its keywords. Same group means at least one author in common.

Same lab group (0)

  • None found.

Different groups (15)

  • 60% Strategies of codon optimization for high-level heterologous protein expression in microbial expression systems (2017)
    Shared keywords: heterologous expression, codon optimization, synthetic gene design, Heterologous, Gene, Codon usage bias · DOI
  • 60% Codon bias and heterologous protein expression (2004)
    Shared keywords: heterologous expression, codon optimization, expression vectors, Heterologous, Gene, Codon usage bias · DOI · PubMed
  • 50% Engineering genes for predictable protein expression (2012)
    Shared keywords: heterologous expression, codon optimization, expression vectors, Heterologous, Gene · DOI · PubMed
  • 50% The Synthetic Gene Designer: a Flexible Web Platform to Explore Sequence Space of Synthetic Genes for Heterologous Expression (2006)
    Shared keywords: heterologous expression, codon optimization, synthetic gene design, Heterologous, Gene · DOI
  • 50% The Synthetic Gene Designer: A flexible web platform to explore sequence manipulation for heterologous expression (2005)
    Shared keywords: heterologous expression, codon optimization, synthetic gene design, Heterologous, Gene · DOI · PubMed
  • 50% Codon adjustment to maximise heterologous gene expression in (1996)
    Shared keywords: heterologous expression, codon optimization, Heterologous, Gene, Gene expression · DOI
  • 40% Optimierung der Expressionsstärke von fremdstoffmetabolisierenden Enzymen in Bakterien und permanenten Zellkulturen für toxikologische Untersuchungen (2006)
    Shared keywords: heterologous expression, codon optimization, Heterologous, Gene
  • 30% Selection on start codons in prokaryotes and potential compensatory nucleotide substitutions (2017)
    Shared keywords: Shine-Dalgarno sequence, Gene, Gene expression · DOI · PubMed
  • 30% Critical reflections on synthetic gene design for recombinant protein expression (2016)
    Shared keywords: synthetic gene design, expression vectors, Gene · DOI · PubMed
  • 30% Roles for Synonymous Codon Usage in Protein Biogenesis (2015)
    Shared keywords: Gene, Codon usage bias, Gene expression · DOI · PubMed
  • 30% Heterologous expression of a lichen polyketide synthase in filamentous fungi (2015)
    Shared keywords: heterologous expression, Heterologous, Gene
  • 30% Multiple roles of the coding sequence 5′ end in gene expression regulation (2014)
    Shared keywords: Gene, Codon usage bias, Gene expression · DOI · PubMed
  • 30% Gene optimization mechanisms: A multi‐gene study reveals a high success rate of full‐length human proteins expressed in Escherichia coli (2010)
    Shared keywords: heterologous expression, Heterologous, Gene · DOI · PubMed
  • 30% Adjustment of Codon Usage Frequencies by Codon Harmonization Improves Protein Expression and Folding (2010)
    Shared keywords: heterologous expression, codon optimization, Heterologous · DOI · PubMed
  • 30% Gene optimization for heterologous expression (2010)
    Shared keywords: heterologous expression, Heterologous, Gene

Cited earlier studies by the same authors

Works in this article's reference list that share at least one author.

None of the cited works share an author with this article.

Authors

ORCID iDs as recorded in OpenAlex.

  1. Anshul Watts ORCID 0000-0003-1014-5302
    Indian Agricultural Research Institute
  2. Subramanian Sankaranarayanan ORCID 0000-0002-0398-2113
    Purdue University West Lafayette
  3. Archana Watts ORCID 0000-0001-6947-2653
    Indian Agricultural Research Institute
  4. Ritesh Kumar Raipuria ORCID 0000-0002-5127-6459

← Assess another article

Feedback